通用引物
象山电视台-探索太空
2023年3月20日发(作者：精子形成过程)Invitrogen中国测序通用引物序列
引物名称序列(5'-3')
M13RCAGGAAACAGCTATGACC
M13FTGTAAAACGACGGCCAGT
M13F(-47)CGCCAGGGTTTTCCCAGTCACGAC
M13R(-48)AGCGGATAACAATTTCACACAGGA
M13(-96)CCCTCATAGTTAGCGTAACG
SP6ATTTAGGTGACACTATAG
T7TAATACGACTCACTATAGGG
T7terminatorTGCTAGTTATTGCTCAGCGG
T3ATTAACCCTCACTAAAGGGA
pGEX-4T-5'GGGCTGGCAAGCCACGTTTGGTG
pGEX-4T-3'CCGGGAGCTGCATGTGTCAGAGG
GLp1TGTATCTTATGGTACTGTAACTG
GLp2CTTTATGTTTTTGGCGTCTTCCA
RVp3CTAGCAAAATAGGCTGTCCC
RVp4GACGATAGTCATGCCCCGCG
pcDNA3.1RTAGAAGGCACAGTCGAGG
PinPointprimerCGTGACGCGGTGCAGGGCG
pCMV-FTCTAAAAGCTGCGGAATTGT
pCMV-RTCCAAACTCATCAATGTATC
pTRC99C-F:TTGCGCCGACATCATAAC
pTRC99C-R:CTGCGTTCTGATTTAATCTG
pCEP-F:AGAGCTCGTTTAGTGAACCG
EBV-R:GTGGTTTGTCCAAACTCATC
pIRES2-EGFP.P5’:GTAGGCGTGTACGGTGGGAG
pIRES2-EGFP.P3’:AACGCACACCGGCCTTATTC
3'AD:AGATGGTGCACGATGCACAG
CMV-FCGCAAATGGGCGGTAGGCGTG
S1CAACGTGAAAAAATTATTATTCGC
S6GTAAATGAATTTTCTGTAGTAGG
5`AOX1GACTGGTTCCAATTGACAAGC
3`AOX1GCAAATGGCATTCTGACATCC
α-FactorTACTATTGCCAGCATTGCTGC
GAL4ADTACCACTACAATGGATG
pACT2-RGTGCACGATGCACAGTTGAA
pB42ADF:CCAGCCTCTTGCTGAGTGGAGATG
pB42ADR:AAGCCGACAACCTTGATTGGAG
pEGFP-N-5’:TGGGAGGTCTATATAAGCAGAG
pEGFP-N-3’:CGTCGCCGTCCAGCTCGACCAG
pEGFP-C-5’:CATGGTCCTGCTGGAGTTCGTG
pEGFP-C-3':TATGGCTGATTATGATCAGT
PBV220F:AAGAAGGGCAGCATTCAAAG
PBV220R:CTGCGTTCTGATTTAATCTG
U6ATGGACTATCATATGCTTACCGTA
2.0revprimer:AGGCGATTAAGTTGGGTA
3.0rev:GAGTTAGCTCACTCATTAGGC
CGCCAGCACATGGAC
5'TriplEx2CTCCGAGATCTGGACGAGC
RVP4GACGATAGTCATGCCCCGCG
RVP3CTAGCAAAATAGGCTGTCCC
pQE30-RGTTCTGAGGTCATTACTGG
pQE30-FTGAGCGGATAACAATTTCAC
pEF-FTCAAGCCTCAGACAGTGGTTC
pDSRED-N1-RTGAAGCGCATGAACTCCTTG
pDsRED-express-C1-FTCCCACAACGAGGACTACAC
pCMV5RATTATAGAGGACACCTAGTC
pCMV5FTTCCAAAATGTCGTAATAAC
P5'TGCGTACTGCGGTGATCAAC
P3'CTGCAAGGCGATTAAGTTGG
BAC2ACGCACAGAATCTAGCGCTT
BAC1AACCATCTCGCAAATAAATA
3'BD:TAAGAGTCACTTTAAAATTTGTATAC
ATTTTCCCAGTCAC
TACACTTTATGCTTC
T7(17base)ACATCCACTTTGCCTTTCTC
TAGAGCGGATGTG
GAL4BDTCATCGGAAGAGAGTAG
TTACTCAAGAACAAGAA
pFlag-CMV-F:GGTAGGCGTGTACGGTGG
pFlag-CMV-R:GCACTGGAGTGGCAACTT
pIRES-F:CTTACTGACATCCACTTTGC
pIRES.R:CACTGCATTCTAGTTGTGGT
P1CCAGGCTTTACACTTTATGC
P2GCGATTAAGTTGGGTAACGC
pLEXA-FCGTCAGCAGAGCTTCACCAT
pLEXA-RTAAAACCTAAGAGTCACTTT
pLNCX-FAGCTCGTTTAGTGAACCGTCAGATCG
pLNCX-RACCTACAGGTGGGGTCTTTCATTCCC
pLXSN-FCTTGAACCTCCTCGTTCGAC
pLXSN-RGTTGCTGACTAATTGAGATG
pSHUTTLE-CMV-FGGTCTATATAAGCAGAGGTG
pSHUTTLE-CMV-RGTGGTATGGCTGATTATGATCAG
MalEPrimerGGTCGTCAGACTGTCGATGAAGCC
Invitrogen中国使用的测序载体和引物
PrimerofVector
VectorPrimer(F)Primer(R)
pACTT7T3
pACT2GAL4ADpACT2-R
pAS2-1GAL4BDpAS2-1.R
pB42ADpB42ADFpB42ADR
pBACPAK8BAC1BAC2
pBK-CMVT7T3
PBS(SK/KS)/M13-M13F/T7T3/M13R
pBV220PBV220FPBV220R
pCAMBIA1301(1300)P1P2
pCAMBIA2300M13R(-48)M13F(-47)
PCANTB5ES1/M13RS6
pCAT3-enhancerRVP3
此载体无反向引物
pcDNA3.0CMV-F/T7SP6/BGH
pcDNA3.1T7BGH
pcDNA4T7/CMV-FBGH
pcDNA6
T7/CMV-F(J21025在T7前面）
BGH
pcDNAIIT7SP6
pCE2.1M13FM13R
pCEP4pCEP-FEBV-R
pCF-TM13FM13R
pCI
T7（17Base）此载体无反向引物
pCI-neo
T7（17Base）
T3
pCMS-EGFPT7T3
pCMV-3Tag-4AT3/PFLAG-CMV-FT7
pCMV5pCMV5FpCMV5R
pCMV5-FlagpCMV5FpCMV5R
pCMV-MYC/HApCMV-FpCMV-R
pCMV-SportM13F/T7SP6/M13R
pCMV-Tag(KAN+)T3T7
pCR2.1-TOPOM13F/T7M13R
pCR3.1T7BGH
pCS2SP6T7
pDonarM13FM13R
pDONR221M13FM13R
pDriveT7SP6
pDRIveR(KAN+)T7SP6
pDsRED1-C1pDsRED-ex-C1-F
pEGFP-N-3’(距离很近,一般
不用)
pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3’
pDSRED-N1(KAN+)pEGFP-N-5’PDSRED-N-R
pECFP-CpEGFP-C-5’pECFP-C-3'
pECFP-N1pEGFP-N-5’pEGFP-N-3’
pEF/myc/ERpEF-FpCDNA3.1R
pEGFP-C(KAN+)pEGFP-C-5’pEGFP-C-3'
pEGFP-N(KAN+)pEGFP-N-5’pEGFP-N-3’
pENTR/D-TOPO(KAN+)M13FM13R
pET-*(His)(KAN+)T7T7Ter
pET22(a,b,c)(AMP+)T7T7Ter
pET28,30,24,49(KAN+)T7T7Ter
pET32(a,b,c)(AMP+)T7/7Ter
pET50(amp+)T7/7TER
pET44(AMP+)T7/7Ter
pEYFP-N1pEGFP-N-5’pEGFP-N-3’
pEYFP-C1pEGFP-C-5’pEGFP-C-3'
pFLAG-CMVpFLAG-CMV-FpFLAG-CMV-R
pGAD424GAL4AD3'AD
pGADGHGAL4AD3'AD
pGADT7-RecGAL4AD/T73'AD
pGAPZa-Aa-FACTOR3'AOX
pGBKT7(Kana+)T73'BD
pGEM-T(-Easy)M13F/T7SP6/M13R
pGEX-(*)TpGEX-4T-5’pGEX-4T-3’
pGL2GLP1GLP2
pGL3RVP3
GLP2(RVP4)(没有特别说明
用GLP2)
PinpointTMVectorPinpointPrimerSP6
pIRESPIRES-F/T7T3/PIRES-R(J40720)
pIRES2-EGFPpIRES2-EGFP.P5’pIRES2-EGFP.P3’
pLXSNpLXSN-FpLXSN-R
pMAL-c2EMalEprimerM13F(-47)
pMAL-C2xP5’P3’
pMAL-p2XMalEprimerM13F(-47)
PMD18-TM13R(-48)M13F(-47)
PMD19-TM13F(-47)M13R(-48)
pPIC9K(AMP+、ZEO+）
5’AOX/a-factor3’AOX
pPICZa5'AOX/PPICZa-F3'AOX
pPROEXHTAM13R(-48)
无反向引物
pQE30or40pQE30-FpQE-R:
pRSETT7T7TER
pSilencer3.1-H1hygroM13F(-47)3.0REV
pSILENCEV2.0-U6T72.0REV
pSK01-TM13FM13R
pSK-CMV(KAN+)T7T3
pSP72T7SP6
pSport1SP6/M13F(-47)T7/M13R
pSUPERT7T3
pTA2
M13F/T7(优先使用M13F）
T3/M13R
pT-AdvM13F/T7M13R
pTARGETTM
pTarget.F(在T7前面）/T7
pTarget.R
pTO-T7T7T7TER
pTRC99a-cpTRC99C-FpTRC99C-R
pTriPLEx25'pTriPLEx2T7
pTWIN-1T7
T7TER(客户确认再用）
pUC18(19)/118(119)M13F(-47)M13R(-48)
pUCm-TM13F/T7M13R
pVAX1T7BGH
pWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)
pXT7T7SP6
pYES2T7pYes2.R
pLEXApLEXA-FpLEXA-R
pLNCXpLNCX-FpLNCX-R
pRECEIVERCMV-F
此载体无反向引物
pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-R
pSP64/65SP6
此载体无反向引物
pSTBlue-1T7SP6
pT7Blue(R)T7M13F(-47)