m13引物
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2023年3月19日发(作者：排球比赛总结)常用之UniversalPrimer序列
PrimerPrimersequenceApplicablevectors
T7TAATACGACTCACTATAGGGpGEM-T,pGEM-T-Easy,pCRII,pET,
pBlueScript,pcDNA3.1,pT7Blue
SP6TATTTAGGTGACACTATAGpGEM-T,pGEM-T-Easy,pCRII
T3ATTAACCCTCACTAAAGGGApBlueScript
pUC/M13Forward(-40)GTTTTCCCAGTCACGACpUC,pGEM-T,pCRII,pBlueScript
pUC/M13Forward(-21)TGTAAAACGACGGCCAGTpUC,pCRII,pBlueScript
pUC/M13ReverseTCACACAGGAAACAGCTATGACpUC,pGEM-T,pCRII,pBlueScript
T7TerminatorGCTAGTTATTGCTCAGCGGpET
pGEX5’GGGCTGGCAAGCCACGTTTGGTGpGEX
pGEX3’CCGGGAGCTGCATGTGTCAGAGGpGEX
pQEFGGCGTATCACGAGGCCCTTTCGpQE
pQERCATTACTGGATCTATCAACAGGpQE
polyhedrinFAAATGATAACCATCTCGCAA
StagGAACGCCAGCACATGGACAGCpET-4x
BGHreverseTAGAAGGCACAGTCGAGGpcDNA3.1,pTracer-CMV
5’AOXGACTGGTTCCAATTGACAAGC
pPlCZ
-factor
TATTGCCAGCATTGCTGC
pPlCZ
3’AOXGCAAATGGCATTCTGACATCC
pPlCZ